5637-CAS9 cell line

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5637-CAS9 cell line 5637-CAS9-产品图
5637-CAS9 Copy Product Info
Catalog#： YC-C102-Cas9-H
Size： 1*10^6
Culture method： 90%RPMI-1640+10%FBS
STR Report
  • Wild-type Cell Lines- 5637-CAS9-Wild-type Cell Lines-
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  • 5637-CAS9 cell line 5637-CAS9-产品图
5637-CAS9 Copy Product Info
Catalog#： YC-C102-Cas9-H
Size： 1*10^6
Culture method： 90%RPMI-1640+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%RPMI-1640+10%FBS
Passage ratio 1:2-1:3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%RPMI-1640+10%FBS
Passage ratio
1:2-1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's 5637 cell line was constructed on the basis of wild-type 5637 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in 5637 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:5637		 STR Info (Cell bank)
Cell Line:5637
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 10111011
D16S539 9999
VWA 18181818
TH01 7979
AMEL XXXY
TPOX 8889
CSF1PO 11111111
D12S391 2020
FGA 2222
D2S1338 2525
D21S11 3636
D18S51 1618
D8S1179 1010
D3S1358 1517
D6S1043 1620
PENTAE 1012
D19S433 1315
PENTAD 1111
D1S1656 1515
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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